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Add new ro-crate exploration tuto #5260

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3 changes: 3 additions & 0 deletions topics/fair/tutorials/earth_system_rocrate/faqs/index.md
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layout: faq-page
---
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138 changes: 138 additions & 0 deletions topics/fair/tutorials/earth_system_rocrate/slides.html
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---
layout: tutorial_slides
logo: "GTN"

title: "An earth-system RO-Crate"
video: true
questions:
- "How to generate a RO-Crate"
- "How to follow a Marine Omics study"
- "How to read a RO-Crate ?"
objectives:
- "Understand RO-Crate in an earth-system context"
time_estimation: 1H
tags:
- earth-system
- ro-crate
- ocean
- marine omics
key_points:
- "RO-Crate in earth-system analysis"
- "Marine omics workflow "
contributions:
authorship:
- Marie59
funding:
- fairease
- eurosciencegateway
---

# Workflow context

The workflow used as an example here goes from :
- First, produce a protein FASTA file from a nucleotide FASTA file using ({% tool [Prodigal](tool_id=toolshed.g2.bx.psu.edu/repos/iuc/prodigal/prodigal/2.6.3+galaxy0) %}) (a tool that predicts protein-coding genes from DNA sequences).
- Then, use ({% tool [InterProscan](toolshed.g2.bx.psu.edu/repos/bgruening/interproscan/interproscan/5.59-91.0+galaxy3) %}) to create a tabular. Interproscan is a batch tool to query the InterPro database. It helps identify and predict the functions of proteins by comparing them to known databases.
- Finally, discover ({% tool [SanntiS](toolshed.g2.bx.psu.edu/repos/ecology/sanntis_marine/sanntis_marine/0.9.3.5+galaxy1) %}) both to build genbank and especially to detect and annotate biosynthetic gene clusters (BGCs).
---

## The workflow itself

<iframe title="Galaxy Workflow Embed" style="width: 100%; height: 700px; border: none;" src="https://earth-system.usegalaxy.eu/published/workflow?id=b9c938d1af08124b&embed=true&buttons=true&about=false&heading=false&minimap=true&zoom_controls=true&initialX=-20&initialY=-20&zoom=1"></iframe>
---

## Run the workflow

Import and run the [Biosynthetic Gene Clusters workflow](https://earth-system.usegalaxy.eu/u/marie.josse/w/marine-omics-identifying-biosynthetic-gene-clusters)

To know more on how to do that follow the [tutorial]({% link topics/ecology/tutorials/marine_omics_bgc/tutorial.md %})
---


## Generate the RO-Crate

Once you run the workflow you can generate the RO-Crate. To do so follow the [tutorial]({% link topics/fair/tutorials/ro-crate-in-galaxy/tutorial.md %})
---


### From workflow to RO-Crate

![From workflow to RO-Crate](./images/workflow_rocrate.png)
---

### The RO-Crate folder and architecture

![From workflow to RO-Crate](./images/all_tree_structure.png)
---

### Let's dive into the ro-crate-metadata.json

![Date of RO-Crate creation and other informations](./images/rocrate1.png)
---

### Description of the RO-Crate structure

![Description of the RO-Crate structure](./images/rocrate2.png)
---

### Galaxy Provenance

![Date of RO-Crate creation and other informations](./images/rocrate3.png)
---

### Title and workflow details

![Title and details of the workflow](./images/rocrate4.png)
---

### Dates

![Dates of running the workflow and creation of the RO-Crate](./images/rocrate5.png)
---

### Data

![Data uploaded for the workflow and produced by it](./images/rocrate6.png)
---

### First tool Prodigal

![Details on Prodigal](./images/rocrate7.png)
---

### Second tool Regex Find and Replace

![Details on Regex Find and Replace](./images/rocrate8.png)
---

### Third tool InterProScan

![Details on InterProScan](./images/rocrate9.png)
---

### Fourth tool SanntiS to build Genbank

![Details on SanntiS to build Genbank](./images/rocrate10.png)
---

### Fourth tool SanntiS to annotate biosynthetic gene clusters

![Details on SanntiS to annotate biosynthetic gene clusters](./images/rocrate11.png)
---

### Details on the different files in the RO-Crate

![Details on the different files in the RO-Crate](./images/rocrate12.png)
---

### End of the ro-crate-metadata.json

![Details on the different files in the RO-Crate and end of the ro-crate-metadata.json](./images/rocrate13.png)
---

### Thank you!

Try to do the same on your workflow!




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