Drop mirror scan step by half

Co-authored-by: Talon Chandler <[email protected]>

mantis/acquisition/settings/example_acquisition_settings.yaml

@@ -32,13 +32,16 @@ lf_slice_settings:
   z_stage_name: 'MCL Piezo'
   z_start: -10
   z_end: 10
-  # When using 0.52 NA transmission light illumination, Nyquist sampling of this
-  # dimension will be achieved by using 300 nm in sample space or by using 420
-  # nm steps of the mirror due to the 1.4x magnification of the remote volume.
-  # Here we chose to oversample the data to match the light-sheet axial
-  # sampling. The 285 nm mirror steps correspond to ~205 nm axial sampling of
-  # the data, matching the light-sheet sampling after deskew and 3x binning.
-  z_step: 0.285
+  # When using 0.52 NA transmission light illumination, Nyquist sampling of the
+  # axial dimension will be achieved by using (450*1.4)/(1.35^2 + 0.52^2) = 300 nm 
+  # steps in sample space. We can achieve this by using (300 nm)*1.4/2 = 210 nm
+  # steps of the mirror due to the 1.4x magnification of the remote volume and the 
+  # fact that the optical path length is extended by twice the mirror's motion. 
+  # 
+  # Here we choose to oversample the data to match the light-sheet axial
+  # sampling. After deskewing and 3x binning, the light-sheet samples are
+  # spaced by ~205 nm, so we choose (205 nm)*1.4/2 = 143 nm mirror steps.
+  z_step: 0.143
   use_sequencing: True
 
 # Define channel settings for the light-sheet acquisition. Channels may have