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put all variant calling parameters together
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charliesimkin authored and alfredsimkin committed Oct 15, 2024
1 parent e12cf95 commit 7ceb096
Showing 1 changed file with 21 additions and 21 deletions.
42 changes: 21 additions & 21 deletions base_resources/user_scripts_and_config/config_v0.5.0.yaml
Original file line number Diff line number Diff line change
Expand Up @@ -82,6 +82,27 @@ wrangler_file: allInfo.tsv.gz
# e.g. pf
species: pf

#This block is for variant calling with freebayes. These are the settings that
#we've found work best for Plasmodium falciparum. If you're using a different
#genome, you may want to alter these settings (see the Jupyter notebook for
#more info)
freebayes_settings:
[
"--pooled-continuous",
"--min-alternate-fraction", "0.01",
"--min-alternate-count", "2",
"--haplotype-length", "3",
"--min-alternate-total", "10",
"--use-best-n-alleles", "70",
"--genotype-qualities",
"--gvcf",
"--gvcf-dont-use-chunk", "true",
]

#freebayes is a particularly memory intensive step. If variant calling is failing
#you might set this to be lower
freebayes_cpu_count: 8

################################################################################
## prevalence calling parameters ##
################################################################################
Expand Down Expand Up @@ -127,27 +148,6 @@ min_wsaf: 0.5
num_samples_umi: 2
min_umi: 3

#This block is for variant calling with freebayes. These are the settings that
#we've found work best for Plasmodium falciparum. If you're using a different
#genome, you may want to alter these settings (see the Jupyter notebook for
#more info)
freebayes_settings:
[
"--pooled-continuous",
"--min-alternate-fraction", "0.01",
"--min-alternate-count", "2",
"--haplotype-length", "3",
"--min-alternate-total", "10",
"--use-best-n-alleles", "70",
"--genotype-qualities",
"--gvcf",
"--gvcf-dont-use-chunk", "true",
]

#freebayes is a particularly memory intensive step. If variant calling is failing
#you might set this to be lower
freebayes_cpu_count: 8

################################################################################
## Advanced Options and Settings ##
################################################################################
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