A pipeline of crossover identification based on scRNA-seq data from gametes, also including analysis of recombination results. Specifically, a project about the recombination of a holocentric plant - R. breviuscula was taken as the instance to delineate the pipeline.
Please cite the following publication when using our pipeline.
Castellani, M., Zhang, M., Thangavel, G., Mata Sucre, Y., Campoy, J. A., Lux, T., ... & Marques, A. (2023). Meiotic recombination dynamics in plants with repeat-based holocentromeres shed light on the primary drivers of crossover patterning. bioRxiv, 2023-04.