uORF-Tools are a workflow and a collection of tools for the analysis of 'upstream Open Reading Frames' (short uORFs). The workflow is based on the workflow management system snakemake and handles installation of all dependencies, as well as all processings steps. The source code of uORF-Tools is open source and available under the GPL-3 License. Installation is described below, for usage please refer to the Userguide.
uORF-Tools requires snakemake (Version=5.4.5), which can be installed with all dependencies via conda. Once you have conda installed simply type:
$ conda create -c conda-forge -c bioconda -n snakemake snakemake==5.4.5
$ source activate
$ source activate snakemake
Create a project directory and change into it:
$ mkdir project
$ cd project
Retrieve the uORF-Tools from GitHub:
$ git clone https://github.com/Biochemistry1-FFM/uORF-Tools.git
Now you can get started. Usage of the workflow is described in the Userguide.