Error in rule run_automatic_analysis

[falsetry1514]

Building DAG of jobs...
Using shell: /bin/bash
Provided cores: 1 (use --cores to define parallelism)
Rules claiming more threads will be scaled down.
Job counts:
count jobs
39 create_automated_analysis_processed_data_files
39 create_automated_report
1 run_analysis
39 run_automated_analysis
118
Select jobs to execute...

[Tue Jul 16 16:53:03 2024]
rule run_automated_analysis:
input: projects/openst_demo/processed_data/e13_mouse_head/illumina/complete_data/dge/dge.all.polyA_adapter_trimmed.mm_included.spatial_beads.mesh_7_hexagon_fc_1_L3_tile_2518.h5ad
output: projects/openst_demo/processed_data/e13_mouse_head/illumina/complete_data/automated_analysis/openst/umi_cutoff_500/e13_mouse_head_fc_1_L3_tile_2518_results.h5ad
jobid: 161
wildcards: project_id=openst_demo, sample_id=e13_mouse_head, data_root_type=complete_data, downsampling_percentage=, run_mode=openst, umi_cutoff=500, puck_barcode_file_id_qc=fc_1_L3_tile_2518

OMP: Info #276: omp_set_nested routine deprecated, please use omp_set_max_active_levels instead.
/Users/server3/spacemake/.snakemake/scripts/tmpmv8a5d3_.automated_analysis.py:84: FutureWarning: In the future, the default backend for leiden will be igraph instead of leidenalg.

To achieve the future defaults please pass: flavor="igraph" and n_iterations=2. directed must also be False to work with igraph's implementation.
sc.tl.leiden(adata, resolution = res, key_added = res_key)
filtering by umi cutoff: 500
starting analysis
normalising and log-scaling
calculating pca components
computing neighborhood graph
dimensionality reduction
clustering
ranking genes for resolution 0.4

0%| | 0/1000 [00:00<?, ?/s]Traceback (most recent call last):
File "", line 1, in
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/spawn.py", line 116, in spawn_main
exitcode = _main(fd, parent_sentinel)
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/spawn.py", line 125, in _main
prepare(preparation_data)
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/spawn.py", line 236, in prepare
_fixup_main_from_path(data['init_main_from_path'])
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/spawn.py", line 287, in _fixup_main_from_path
main_content = runpy.run_path(main_path,
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/runpy.py", line 289, in run_path
return _run_module_code(code, init_globals, run_name,
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/runpy.py", line 96, in _run_module_code
_run_code(code, mod_globals, init_globals,
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/runpy.py", line 86, in run_code
exec(code, run_globals)
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/spacemake/snakemake/scripts/automated_analysis.py", line 9, in
adata = sc.read_h5ad(snakemake.input[0])
NameError: name 'snakemake' is not defined
Traceback (most recent call last):
File "/Users/server3/spacemake/.snakemake/scripts/tmpmv8a5d3.automated_analysis.py", line 92, in
sq.gr.nhood_enrichment(adata, cluster_key=res_key)
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/squidpy/gr/_nhood.py", line 180, in nhood_enrichment
perms = parallelize(
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/squidpy/_utils.py", line 169, in wrapper
queue = Manager().Queue()
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/context.py", line 57, in Manager
m.start()
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/managers.py", line 566, in start
self._address = reader.recv()
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/connection.py", line 250, in recv
buf = self._recv_bytes()
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/connection.py", line 414, in _recv_bytes
buf = self._recv(4)
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/multiprocessing/connection.py", line 383, in _recv
raise EOFError
EOFError

0%| | 0/1000 [00:05<?, ?/s]
[Tue Jul 16 16:53:29 2024]
Error in rule run_automated_analysis:
jobid: 161
output: projects/openst_demo/processed_data/e13_mouse_head/illumina/complete_data/automated_analysis/openst/umi_cutoff_500/e13_mouse_head_fc_1_L3_tile_2518_results.h5ad

RuleException:
CalledProcessError in line 665 of /Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/spacemake/snakemake/main.smk:
Command 'set +o pipefail; JAVA_TOOL_OPTIONS="-Xmx8g -Xss2560k" ; /Users/server3/miniforge3/envs/spacemake/bin/python3.10 /Users/server3/spacemake/.snakemake/scripts/tmpmv8a5d3_.automated_analysis.py' returned non-zero exit status 1.
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/snakemake/executors/init.py", line 2330, in run_wrapper
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/spacemake/snakemake/main.smk", line 665, in __rule_run_automated_analysis
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/snakemake/executors/init.py", line 569, in _callback
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/concurrent/futures/thread.py", line 58, in run
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/snakemake/executors/init.py", line 555, in cached_or_run
File "/Users/server3/miniforge3/envs/spacemake/lib/python3.10/site-packages/snakemake/executors/init.py", line 2362, in run_wrapper
Shutting down, this might take some time.
Exiting because a job execution failed. Look above for error message
output provided by 'mapping.smk' module (via 'get_mapped_BAM_output'): 'projects/openst_demo/processed_data/e13_mouse_head/illumina/complete_data/final.polyA_adapter_trimmed.bam'

I am sured that this py file has run in process, the shell print these lines:
shell:
filtering by umi cutoff: 500
starting analysis
normalising and log-scaling
calculating pca components
computing neighborhood graph
dimensionality reduction
clustering
ranking genes for resolution 0.4

But it still report an error.
it maybe report an error at sc.tl.rank_genes_groups() or sq.gr.nhood_enrichment(). Please tell me how to deal with this problem, I almost run this program successfully on my Mac. The other problems before this have solved, I'm eager to make it success.