Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Support slow lamps #363

Open
talonchandler opened this issue May 5, 2023 · 0 comments
Open

Support slow lamps #363

talonchandler opened this issue May 5, 2023 · 0 comments

Comments

@talonchandler
Copy link
Collaborator

I tried to install recOrder on a system with a slow (~1.5 second ramp-up) halogen lamp and no shutter, and I found that the default behavior caused a lot of difficulty and failed reconstructions.

Since I didn't have a shutter available, I connected micromanager's "shutter" to the lamp. This gave recOrder control over the lamp, and it started the lamp when I requested an acquisition. Since the lamp was very slow, the first state with very underexposed, the second state was slightly underexposed, and the final states were nearly nominal. These acquisition gave completely incorrect reconstructions.

My workaround was to turn on live mode ahead of an acquisition, but, annoyingly, recOrder would turn off the live mode when the acquisition finished so I had to remember to turn on live mode to boot up the lamp every time.

I think a good solution will involve:

  • documenting how to setup a slow shutterless lamp in micromanager so that it's compatible with recOrder (setting and testing delay times...I didn't have time to set this up properly)
  • having recOrder reinstate the "Live" or "not Live" mode after an acquisition. The fact that recOrder turned off live mode was an unnecessary inconvenience.
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant