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I tried to install recOrder on a system with a slow (~1.5 second ramp-up) halogen lamp and no shutter, and I found that the default behavior caused a lot of difficulty and failed reconstructions.
Since I didn't have a shutter available, I connected micromanager's "shutter" to the lamp. This gave recOrder control over the lamp, and it started the lamp when I requested an acquisition. Since the lamp was very slow, the first state with very underexposed, the second state was slightly underexposed, and the final states were nearly nominal. These acquisition gave completely incorrect reconstructions.
My workaround was to turn on live mode ahead of an acquisition, but, annoyingly, recOrder would turn off the live mode when the acquisition finished so I had to remember to turn on live mode to boot up the lamp every time.
I think a good solution will involve:
documenting how to setup a slow shutterless lamp in micromanager so that it's compatible with recOrder (setting and testing delay times...I didn't have time to set this up properly)
having recOrder reinstate the "Live" or "not Live" mode after an acquisition. The fact that recOrder turned off live mode was an unnecessary inconvenience.
The text was updated successfully, but these errors were encountered:
I tried to install recOrder on a system with a slow (~1.5 second ramp-up) halogen lamp and no shutter, and I found that the default behavior caused a lot of difficulty and failed reconstructions.
Since I didn't have a shutter available, I connected micromanager's "shutter" to the lamp. This gave recOrder control over the lamp, and it started the lamp when I requested an acquisition. Since the lamp was very slow, the first state with very underexposed, the second state was slightly underexposed, and the final states were nearly nominal. These acquisition gave completely incorrect reconstructions.
My workaround was to turn on live mode ahead of an acquisition, but, annoyingly, recOrder would turn off the live mode when the acquisition finished so I had to remember to turn on live mode to boot up the lamp every time.
I think a good solution will involve:
The text was updated successfully, but these errors were encountered: