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I am using HERRO-corrected R9.4 data. It originally has a coverage of 450x and, after filtering for a min-length of 10kbp and error correction, it is down to 240x. Then, I take a subset of it with 35x coverage to use as the main input to the Hifiasm.
HERRO-corrected R9 reads were shown to be usable at around 30-35x coverage by the original HERRO paper. I use them with the raw ultra-long reads longer than 50kbp as the inputs to the Hifiasm.
However, assemblies are poor and the k-mer counts I have below are also interesting, what kind of a solution (such as parameter sweeps) do I need?
Hi,
I am using HERRO-corrected R9.4 data. It originally has a coverage of 450x and, after filtering for a min-length of 10kbp and error correction, it is down to 240x. Then, I take a subset of it with 35x coverage to use as the main input to the Hifiasm.
HERRO-corrected R9 reads were shown to be usable at around 30-35x coverage by the original HERRO paper. I use them with the raw ultra-long reads longer than 50kbp as the inputs to the Hifiasm.
However, assemblies are poor and the k-mer counts I have below are also interesting, what kind of a solution (such as parameter sweeps) do I need?
[M::ha_analyze_count] lowest: count[55] = 3749
[M::ha_analyze_count] highest: count[228] = 38565
[M::ha_hist_line] 2: ****************************************************************************************************> 45448636
[M::ha_hist_line] 3: ****************************************************************************************************> 16128660
[M::ha_hist_line] 4: ****************************************************************************************************> 7874831
[M::ha_hist_line] 5: ****************************************************************************************************> 4473368
[M::ha_hist_line] 6: ****************************************************************************************************> 2780220
[M::ha_hist_line] 7: ****************************************************************************************************> 1850257
[M::ha_hist_line] 8: ****************************************************************************************************> 1286884
[M::ha_hist_line] 9: ****************************************************************************************************> 929670
[M::ha_hist_line] 10: ****************************************************************************************************> 696630
[M::ha_hist_line] 11: ****************************************************************************************************> 530947
[M::ha_hist_line] 12: ****************************************************************************************************> 414721
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[M::ha_hist_line] 14: ****************************************************************************************************> 263659
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[M::ha_hist_line] 18: ****************************************************************************************************> 121472
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[M::ha_hist_line] 20: ****************************************************************************************************> 87593
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[M::ha_hist_line] 22: ****************************************************************************************************> 65062
[M::ha_hist_line] 23: ****************************************************************************************************> 55768
[M::ha_hist_line] 24: ****************************************************************************************************> 48143
[M::ha_hist_line] 25: ****************************************************************************************************> 42026
[M::ha_hist_line] 26: ************************************************************************************************** 37878
[M::ha_hist_line] 27: ************************************************************************************** 33327
[M::ha_hist_line] 28: **************************************************************************** 29335
[M::ha_hist_line] 29: ******************************************************************** 26116
[M::ha_hist_line] 30: ************************************************************* 23485
[M::ha_hist_line] 31: ****************************************************** 20697
[M::ha_hist_line] 32: ************************************************* 18771
[M::ha_hist_line] 33: ******************************************** 16944
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[M::ha_hist_line] 35: ************************************ 14044
[M::ha_hist_line] 36: ********************************** 12958
[M::ha_hist_line] 37: ****************************** 11644
[M::ha_hist_line] 38: **************************** 10761
[M::ha_hist_line] 39: ************************** 9862
[M::ha_hist_line] 40: ************************ 9271
[M::ha_hist_line] 41: ********************** 8385
[M::ha_hist_line] 42: ********************* 7960
[M::ha_hist_line] 43: ******************* 7326
[M::ha_hist_line] 44: ****************** 6847
[M::ha_hist_line] 45: **************** 6297
[M::ha_hist_line] 46: *************** 5842
[M::ha_hist_line] 47: ************** 5462
[M::ha_hist_line] 48: ************** 5262
[M::ha_hist_line] 49: ************* 5115
[M::ha_hist_line] 50: ************* 4880
[M::ha_hist_line] 51: ************ 4713
[M::ha_hist_line] 52: ************ 4470
[M::ha_hist_line] 53: *********** 4256
[M::ha_hist_line] 54: ********** 4023
[M::ha_hist_line] 55: ********** 3749
[M::ha_hist_line] 56: ********** 3780
[M::ha_hist_line] 57: ********** 3678
[M::ha_hist_line] 58: ********* 3629
[M::ha_hist_line] 59: ******** 3237
[M::ha_hist_line] 60: ******** 3188
[M::ha_hist_line] 61: ******** 3090
[M::ha_hist_line] 62: ******** 3050
[M::ha_hist_line] 63: ******** 2964
[M::ha_hist_line] 64: ******* 2862
[M::ha_hist_line] 65: ******* 2701
[M::ha_hist_line] 66: ******* 2707
[M::ha_hist_line] 67: ****** 2494
[M::ha_hist_line] 68: ****** 2478
[M::ha_hist_line] 69: ****** 2405
[M::ha_hist_line] 70: ****** 2400
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[M::ha_hist_line] 72: ****** 2202
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[M::ha_hist_line] 74: ***** 1952
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[M::ha_hist_line] 108: ***** 1932
[M::ha_hist_line] 109: ***** 1993
[M::ha_hist_line] 110: ***** 2013
[M::ha_hist_line] 111: ****** 2206
[M::ha_hist_line] 112: ****** 2419
[M::ha_hist_line] 113: ****** 2488
[M::ha_hist_line] 114: ******* 2528
[M::ha_hist_line] 115: ******* 2604
[M::ha_hist_line] 116: ******* 2710
[M::ha_hist_line] 117: ******* 2836
[M::ha_hist_line] 118: ******** 2962
[M::ha_hist_line] 119: ******** 3012
[M::ha_hist_line] 120: ******** 3042
[M::ha_hist_line] 121: ******** 3237
[M::ha_hist_line] 122: ********* 3507
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[M::ha_hist_line] 138: **************** 6116
[M::ha_hist_line] 139: **************** 6267
[M::ha_hist_line] 140: **************** 6330
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[M::ha_hist_line] 146: ******************** 7752
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[M::ha_hist_line] 149: ********************** 8454
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[M::ha_hist_line] 152: ************************ 9139
[M::ha_hist_line] 153: ************************ 9368
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[M::ha_hist_line] 164: ******************************** 12217
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[M::ha_hist_line] 188: ************************************************************* 23477
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[M::ha_hist_line] 190: **************************************************************** 24619
[M::ha_hist_line] 191: ***************************************************************** 25133
[M::ha_hist_line] 192: ******************************************************************* 25698
[M::ha_hist_line] 193: ******************************************************************* 25955
[M::ha_hist_line] 194: ********************************************************************* 26793
[M::ha_hist_line] 195: ********************************************************************** 26882
[M::ha_hist_line] 196: *********************************************************************** 27565
[M::ha_hist_line] 197: ************************************************************************ 27888
[M::ha_hist_line] 198: ************************************************************************** 28600
[M::ha_hist_line] 199: **************************************************************************** 29369
[M::ha_hist_line] 200: ***************************************************************************** 29609
[M::ha_hist_line] 201: ****************************************************************************** 30215
[M::ha_hist_line] 202: ******************************************************************************* 30442
[M::ha_hist_line] 203: ********************************************************************************* 31240
[M::ha_hist_line] 204: ********************************************************************************** 31791
[M::ha_hist_line] 205: *********************************************************************************** 31877
[M::ha_hist_line] 206: ************************************************************************************ 32380
[M::ha_hist_line] 207: *************************************************************************************** 33445
[M::ha_hist_line] 208: **************************************************************************************** 33927
[M::ha_hist_line] 209: ***************************************************************************************** 34170
[M::ha_hist_line] 210: ****************************************************************************************** 34695
[M::ha_hist_line] 211: ******************************************************************************************** 35413
[M::ha_hist_line] 212: ******************************************************************************************* 35078
[M::ha_hist_line] 213: ******************************************************************************************** 35622
[M::ha_hist_line] 214: ******************************************************************************************** 35650
[M::ha_hist_line] 215: ********************************************************************************************** 36145
[M::ha_hist_line] 216: ************************************************************************************************ 36950
[M::ha_hist_line] 217: *********************************************************************************************** 36804
[M::ha_hist_line] 218: ************************************************************************************************* 37238
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[M::ha_hist_line] 221: *************************************************************************************************** 38081
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[M::ha_hist_line] 224: ************************************************************************************************** 37935
[M::ha_hist_line] 225: *************************************************************************************************** 38051
[M::ha_hist_line] 226: *************************************************************************************************** 38064
[M::ha_hist_line] 227: **************************************************************************************************** 38489
[M::ha_hist_line] 228: **************************************************************************************************** 38565
[M::ha_hist_line] 229: **************************************************************************************************** 38388
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[M::ha_hist_line] 233: ************************************************************************************************** 37895
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[M::ha_hist_line] 235: ************************************************************************************************** 37892
[M::ha_hist_line] 236: ************************************************************************************************* 37580
[M::ha_hist_line] 237: ************************************************************************************************* 37462
[M::ha_hist_line] 238: *********************************************************************************************** 36751
[M::ha_hist_line] 239: *********************************************************************************************** 36570
[M::ha_hist_line] 240: ********************************************************************************************** 36273
[M::ha_hist_line] 241: ******************************************************************************************** 35646
[M::ha_hist_line] 242: ******************************************************************************************** 35590
[M::ha_hist_line] 243: ******************************************************************************************* 34987
[M::ha_hist_line] 244: ****************************************************************************************** 34594
[M::ha_hist_line] 245: **************************************************************************************** 33839
[M::ha_hist_line] 246: ************************************************************************************** 33221
[M::ha_hist_line] 247: ************************************************************************************** 33033
[M::ha_hist_line] 248: ************************************************************************************ 32564
[M::ha_hist_line] 249: *********************************************************************************** 32017
[M::ha_hist_line] 250: ********************************************************************************** 31585
[M::ha_hist_line] 251: ******************************************************************************** 31026
[M::ha_hist_line] 252: ****************************************************************************** 30157
[M::ha_hist_line] 253: ***************************************************************************** 29693
[M::ha_hist_line] 254: *************************************************************************** 29114
[M::ha_hist_line] 255: ************************************************************************** 28394
[M::ha_hist_line] 256: ************************************************************************ 27674
[M::ha_hist_line] 257: ********************************************************************** 26806
[M::ha_hist_line] 258: ********************************************************************* 26420
[M::ha_hist_line] 259: ******************************************************************* 25779
[M::ha_hist_line] 260: ***************************************************************** 24996
[M::ha_hist_line] 261: **************************************************************** 24691
[M::ha_hist_line] 262: ************************************************************* 23562
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[M::ha_hist_line] 269: ************************************************* 18870
[M::ha_hist_line] 270: ************************************************ 18355
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[M::ha_hist_line] 277: *********************************** 13610
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[M::ha_hist_line] 280: ******************************* 11945
[M::ha_hist_line] 281: ****************************** 11577
[M::ha_hist_line] 282: ***************************** 11007
[M::ha_hist_line] 283: *************************** 10559
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[M::ha_hist_line] 286: *********************** 9025
[M::ha_hist_line] 287: ********************** 8613
[M::ha_hist_line] 288: ********************* 8185
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[M::ha_hist_line] 293: **************** 6285
[M::ha_hist_line] 294: *************** 5761
[M::ha_hist_line] 295: ************** 5589
[M::ha_hist_line] 296: ************** 5286
[M::ha_hist_line] 297: ************* 4833
[M::ha_hist_line] 298: ************ 4647
[M::ha_hist_line] 299: *********** 4255
[M::ha_hist_line] 300: *********** 4089
[M::ha_hist_line] 301: ********** 3797
[M::ha_hist_line] 302: ********* 3529
[M::ha_hist_line] 303: ******** 3269
[M::ha_hist_line] 304: ******** 3080
[M::ha_hist_line] 305: ******* 2837
[M::ha_hist_line] 306: ******* 2664
[M::ha_hist_line] 307: ****** 2473
[M::ha_hist_line] 308: ****** 2289
[M::ha_hist_line] 309: ****** 2172
[M::ha_hist_line] 310: ***** 1978
[M::ha_hist_line] 311: ***** 1852
[M::ha_hist_line] 312: ***** 1772
[M::ha_hist_line] 313: **** 1606
[M::ha_hist_line] 314: **** 1471
[M::ha_hist_line] 315: **** 1402
[M::ha_hist_line] 316: *** 1233
[M::ha_hist_line] 317: *** 1127
[M::ha_hist_line] 318: *** 1082
[M::ha_hist_line] 319: ** 959
[M::ha_hist_line] 320: ** 948
[M::ha_hist_line] 321: ** 793
[M::ha_hist_line] 322: ** 807
[M::ha_hist_line] 323: ** 733
[M::ha_hist_line] 324: ** 657
[M::ha_hist_line] 325: ** 590
[M::ha_hist_line] 326: * 534
[M::ha_hist_line] 327: * 496
[M::ha_hist_line] 328: * 472
[M::ha_hist_line] 329: * 471
[M::ha_hist_line] 330: * 413
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[M::ha_hist_line] 332: * 342
[M::ha_hist_line] 333: * 308
[M::ha_hist_line] 334: * 300
[M::ha_hist_line] 335: * 259
[M::ha_hist_line] 336: * 259
[M::ha_hist_line] 337: * 220
[M::ha_hist_line] 338: * 245
[M::ha_hist_line] 339: * 199
[M::ha_hist_line] 340: * 200
[M::ha_hist_line] rest: ********************************************************************************************* 35872
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