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Intensity of optogenetic stimulus? #6

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CodyCBakerPhD opened this issue Apr 1, 2024 · 4 comments
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Intensity of optogenetic stimulus? #6

CodyCBakerPhD opened this issue Apr 1, 2024 · 4 comments
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@CodyCBakerPhD
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Details are given for intensity of the calcium imaging in the methods section, but not for the optogenetic stimulation

@CodyCBakerPhD CodyCBakerPhD self-assigned this Apr 1, 2024
@emosb
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emosb commented Apr 1, 2024

I can measure this to check that it is still accurate, but in the methods of the Randi paper it is reported that "the power of the laser at the sample was 1.2 mW at 500 kHz" which is referring to the optogenetic stimulus laser pulse

@CodyCBakerPhD
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CodyCBakerPhD commented Apr 1, 2024

@emosb That is the power, which I am indeed using - the intensity would be something in mW/mm^2, like in the paragraph from the methods section

For functional connectivity experiments, an intensity of 1.4 mW mm−2 at the sample plane was used to image GCaMP6s...

@CodyCBakerPhD
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@emosb Unless you're saying the intensity is the same in the ogen case as in the imaging case because it's the same laser?

@CodyCBakerPhD
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Leaving this empty since it would be too complicated and not worthwhile to report

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