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Provision of enzymes #349

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molinfzl opened this issue May 9, 2024 · 0 comments
Open

Provision of enzymes #349

molinfzl opened this issue May 9, 2024 · 0 comments

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@molinfzl
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molinfzl commented May 9, 2024

my command is : ./juicer.sh -z ./ga.fasta -p ./ga.length -y ./ga_MboI.txt -d /home/ga -D /home/software/juicer -g ga -s MboI -t 40

My question is that when I download data from the Internet, I often do not know what endonuclease is used, so can I tell from the output of this command whether the name of the enzyme I selected is correct.

Output: inter_30.txt

Sequenced Read Pairs: 395,348,657
Normal Paired: 178,269,979 (45.09%)
Chimeric Paired: 193,107,567 (48.84%)
Chimeric Ambiguous: 17,990,761 (4.55%)
Unmapped: 5,980,350 (1.51%)
Ligation Motif Present: 128,140 (0.03%)
Alignable (Normal+Chimeric Paired): 371,377,546 (93.94%)
Unique Reads: 323,068,010 (81.72%)
PCR Duplicates: 48,309,536 (12.22%)
Optical Duplicates: 0 (0.00%)
Library Complexity Estimate: 1,300,794,258

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