Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

More restriction enzyme sites of Arima than Juicer provided, how to modified juicer.sh #332

Open
QianghuiZhu opened this issue Sep 26, 2023 · 4 comments
Labels

Comments

@QianghuiZhu
Copy link

Hi!
Juicer + 3D-DNA + juicebox is a great pipeline.
I have run successfully for DpnII. But for Arima, I have some questions.
I downloaded Hi-C data from VGP, and their Arima provided four restriction enzyme sites (GATC,GANTC,CTNAG,TTAA). But I found that generate_site_positions.py and juicer.sh only inculde two of these sites.
image
1695715985832

So, My questions are:
Q1: May I modify generate_site_positions.py line 56 to "'Arima' : [ 'GATC', 'GANTC', 'CTNAG', 'TTAA' ]," to contain all of four sites?
Q2: If Q1 is yes, what should I modify in juicer.sh?
Q3: Any other need to be modified or noticed?

Best!

@AlcaArctica
Copy link

I have the same question. Has there been any resolution?

@QianghuiZhu
Copy link
Author

I have the same question. Has there been any resolution?

It seems that SALSA2 could provide four restriction enzyme sites.

@sa501428
Copy link
Member

Hi! Yes you can edit it and submit a pull request. (And of course, you can always edit downloaded code internally)

@QianghuiZhu
Copy link
Author

Hi! Yes you can edit it and submit a pull request. (And of course, you can always edit downloaded code internally)

Thanks for this.
But I do not know where should be edit. I do not clear that how to modified juicer.sh and other scripts.
Best!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
Projects
None yet
Development

No branches or pull requests

3 participants